What are the detection and diagnosis methods for Candida infection
The most common cause of vaginitis is fungus Candida albicans infection. Candida is a common strain in the vagina. Before and after menstruation or labor, when the normal balance between mold and bacteria is disrupted, the number of candida increases rapidly g spot vibrator , leading to infection. Candida albicans does not cause symptoms and can exist in the human mouth penis vibrator , intestines, and vaginal mucosa.
Diagnosis of candidiasis:
1。Direct microscopy: Women use a long disinfectant cotton swab to collect vaginal gay masturbation , cervical secretions or milky white films on the vaginal wall, and men scrape scales on the surface of skin lesions on the glans of the penis, coronary sulcus or prepuce as specimens to be examined. Make 10% of the specimen to be inspected with potassium hydroxide or physiological saline. Under the microscope, groups of ospores and pseudohyphae can be seen. If many pseudohyphae are found, it means that Candida is in the disease stage, which is more useful for diagnosis. meaningful.
2。Staining examination: Gram staining, Congo red staining or PAS staining staining showed that the positive rate was higher than that of direct microscopy. Gram stain, spores and pseudohyphae dye blue: Congo red and PAS stain, spores and pseudohyphae dye red.
3。Isolation and culture: Patients with negative coating test can undergo Candida culture. Inoculate the test specimens into Sabouraud culture under sterile conditions (usually using tube culture). When inoculating, cut off the test tube culture medium obliquely a little cock pump , inoculate 2 to 3 places per tube, and inoculate 2 tubes per specimen. Place the culture medium in a 37℃ incubator and incubate for 24-48 hours, and observe it. A large number of milky white colonies can be seen growing. Use an inoculation needle to pick up a small number of colony smears, and conduct direct microscopy or microscopic microscopy after staining. A large number of blastospores can be seen, which can be initially diagnosed as Candida infection.
Four. Antibodies to Candida albicans can be detected by double immunodiffusion or latex coagulation.
Diagnosis of candidiasis:
1。Direct microscopy: Women use a long disinfectant cotton swab to collect vaginal gay masturbation , cervical secretions or milky white films on the vaginal wall, and men scrape scales on the surface of skin lesions on the glans of the penis, coronary sulcus or prepuce as specimens to be examined. Make 10% of the specimen to be inspected with potassium hydroxide or physiological saline. Under the microscope, groups of ospores and pseudohyphae can be seen. If many pseudohyphae are found, it means that Candida is in the disease stage, which is more useful for diagnosis. meaningful.
2。Staining examination: Gram staining, Congo red staining or PAS staining staining showed that the positive rate was higher than that of direct microscopy. Gram stain, spores and pseudohyphae dye blue: Congo red and PAS stain, spores and pseudohyphae dye red.
3。Isolation and culture: Patients with negative coating test can undergo Candida culture. Inoculate the test specimens into Sabouraud culture under sterile conditions (usually using tube culture). When inoculating, cut off the test tube culture medium obliquely a little cock pump , inoculate 2 to 3 places per tube, and inoculate 2 tubes per specimen. Place the culture medium in a 37℃ incubator and incubate for 24-48 hours, and observe it. A large number of milky white colonies can be seen growing. Use an inoculation needle to pick up a small number of colony smears, and conduct direct microscopy or microscopic microscopy after staining. A large number of blastospores can be seen, which can be initially diagnosed as Candida infection.
Four. Antibodies to Candida albicans can be detected by double immunodiffusion or latex coagulation.
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