How to check and diagnose candidiasis
Candida albicans infection is mainly manifested as vulva itching, burning pain. In severe cases, bedridden, accompanied by frequent urination, painful urine biggest dildo , and painful sexual intercourse. In the acute period, the leucorrhea is increased. The leucorrhea is characterized by thick white color and curd or bean dregs. Inspect the vulva scratches. There is white membrane on the inner side of the labia minora and the vaginal mucosa. After wiping, red and swollen mucosa surfaces appear. Erosion and superficial ulcers may also appear in the acute period.
Diagnosis of candidiasis:
1。Direct microscopy: Women use a long disinfectant cotton wipe to remove vaginal, cervical secretions or milky white films on vaginal wall ed vacuum pump vacuum pumps , and men take scales on the surface of the penis glans, coronary sulcus or prepuce injury as specimens to be examined. Make 10% of the specimen to be inspected with potassium hydroxide or physiological saline. Under the microscope, groups of ospores and pseudohyphae can be seen. If many pseudohyphae are found, it means that Candida is in the disease stage, which is more useful for diagnosis. meaningful.
2。Staining inspection: Gram staining, Congo red staining or PAS staining can also be used before staining and microscopic examination. The positive rate is higher than that of direct microscopic examination. Gram stain, spores and pseudohyphae dye blue: Congo red and PAS stain, spores and pseudohyphae dye red.
3。Isolation and culture: Patients with negative coating test can undergo Candida culture. Inoculate the test specimens into Sabouraud culture under sterile conditions (usually using tube culture). When inoculating, cut off the test tube culture medium obliquely a little, inoculate 2 to 3 places per tube, and inoculate 2 tubes per specimen. Place the culture medium in an incubator at 37℃ for incubation for 24-48 hours and observe it. A large number of milky white colonies grow. Use an inoculation needle to select a small number of colony smears vibrator for women , and directly conduct microscopic examination or staining microscopic examination. A large number of bacterial spores can be seen. The preliminary diagnosis is Candida infection.
4。Antibodies to Candida albicans can be detected by double immunodiffusion method or latex coagulation method.
Diagnosis of candidiasis:
1。Direct microscopy: Women use a long disinfectant cotton wipe to remove vaginal, cervical secretions or milky white films on vaginal wall ed vacuum pump vacuum pumps , and men take scales on the surface of the penis glans, coronary sulcus or prepuce injury as specimens to be examined. Make 10% of the specimen to be inspected with potassium hydroxide or physiological saline. Under the microscope, groups of ospores and pseudohyphae can be seen. If many pseudohyphae are found, it means that Candida is in the disease stage, which is more useful for diagnosis. meaningful.
2。Staining inspection: Gram staining, Congo red staining or PAS staining can also be used before staining and microscopic examination. The positive rate is higher than that of direct microscopic examination. Gram stain, spores and pseudohyphae dye blue: Congo red and PAS stain, spores and pseudohyphae dye red.
3。Isolation and culture: Patients with negative coating test can undergo Candida culture. Inoculate the test specimens into Sabouraud culture under sterile conditions (usually using tube culture). When inoculating, cut off the test tube culture medium obliquely a little, inoculate 2 to 3 places per tube, and inoculate 2 tubes per specimen. Place the culture medium in an incubator at 37℃ for incubation for 24-48 hours and observe it. A large number of milky white colonies grow. Use an inoculation needle to select a small number of colony smears vibrator for women , and directly conduct microscopic examination or staining microscopic examination. A large number of bacterial spores can be seen. The preliminary diagnosis is Candida infection.
4。Antibodies to Candida albicans can be detected by double immunodiffusion method or latex coagulation method.
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